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                                                                   	                              	                               	                                             	                                    	                                    	               ;   6         
            7                       
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
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                         	          	           	               	            	            	     ; 6   
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h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W=  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ‡<?xml version="1.0"?><Features nextValidID="14"><Feature recentID="13" name="Ub-K27" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="883-1113" color="#ffcc99" type="standard"/></Feature><Feature recentID="12" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3978-3994" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1402-1418" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1378-1394" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1471-1492" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1113" maxFusedRunOn="1113" detectionMode="exactProteinMatch"><Segment range="832-858" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment name="-10" range="1426-1432" color="#ffffff" type="standard"/><Segment range="1433-1450" color="#ffffff" type="standard"/><Segment name="-35" range="1451-1456" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; 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   A   
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  \<Notes>
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<CreatedBy>Huanghua</CreatedBy>
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<LastModified UTC="4:49:37">2021.8.9</LastModified>
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<CreatedBy>Huanghua</CreatedBy>
<SequenceClass>UNA</SequenceClass>
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  \<Notes>
<UUID>298ceb86-6864-4280-a605-324a97befbe0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2016.6.28</Created>
<LastModified UTC="4:47:24">2021.8.9</LastModified>
<CreatedBy>Huanghua</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>DH5Î±â„¢</TransformedInto>
</Notes>
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   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ´<Notes>
<UUID>22c3c964-92c2-4fc1-9402-ef5e33f2eb58</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCMV-HA-UB-K27</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2016.6.28</Created>
<LastModified UTC="3:13:0">2025.9.4</LastModified>
<CreatedBy>Huanghua</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>DH5Î±â„¢</TransformedInto>
</Notes>
  Y     K         
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   )<?xml version="1.0"?><CustomEnzymeSets/>
